multiphor ii apparatus (Danaher Inc)

Danaher Inc is a verified supplier

Danaher Inc manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Danaher Inc multiphor ii apparatus
Multiphor Ii Apparatus, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 92/100, based on 1006 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multiphor+ii+apparatus/Smart-Max+AS-310+device/pmc00333412-62-53-56
Average 92 stars, based on 1006 article reviews

multiphor ii apparatus - by Bioz Stars, 2026-11

92/100 stars

Images

Incubation:

Article Title: Reproductive development of Santa Inês rams during the first year of life: body and testis growth, testosterone concentrations, sperm parameters, age at puberty and seminal plasma proteins.
Article Snippet: We have investigated the reproductive development of the tropically adapted Santa Inês ram, the most common hair sheep in Brazil.. From 8 to 48 weeks of age, 16 animals were evaluated for body and testis growth, semen parameters, testosterone concentrations and seminal plasma proteins, using two-dimensional SDS-PAGE.. Animals were weaned at 30 days and kept in feedlots thereafter, receiving hay, concentrate (18% of crude protein) and mineral supplement.

Article Title: Electrophoretic characterization of species of fibronectin bearing sequences from the N-terminal heparin-binding domain in synovial fluid samples from patients with osteoarthritis and rheumatoid arthritis
Article Snippet: The weights of molecular standards (Gibco BRL, Rockville, MD, USA) are those reported by the manufacturer for prestained proteins. .. Dithiothreitol (18.2 mM), PMSF (2 mM), and aprotinin (0.1 U/ml) were added to the rehydration solution just before the sample, and the mixture was centrifuged at 14,000 g for 15 min and applied via sample cup to a 7-cm isoelectric focusing strip (pre-equilibrated overnight in rehydration solution) for focusing at 20°C in a Multiphor II apparatus (Amersham Pharmacia) at 200 V for 1 min followed by 3500 V for 170 min. After storage at -75°C, strips were incubated for 15 min in 50 mM Tris, pH 8.8, plus 6 M urea, 30% glycerol, 2% SDS, trimmed to exclude ~7 mm from the anodic end, and submitted to 5% SDS–PAGE with an overlay of 0.5% agarose in 25 mM Tris, 192 mM glycine, and 0.1% SDS. .. Dithiothreitol (18.2 mM), PMSF (2 mM), and aprotinin (0.1 U/ml) were added to the rehydration solution just before the sample, and the mixture was centrifuged at 14,000 g for 15 min and applied via sample cup to a 7-cm isoelectric focusing strip (pre-equilibrated overnight in rehydration solution) for focusing at 20°C in a Multiphor II apparatus (Amersham Pharmacia) at 200 V for 1 min followed by 3500 V for 170 min. After storage at -75°C, strips were incubated for 15 min in 50 mM Tris, pH 8.8, plus 6 M urea, 30% glycerol, 2% SDS, trimmed to exclude ~7 mm from the anodic end, and submitted to 5% SDS–PAGE with an overlay of 0.5% agarose in 25 mM Tris, 192 mM glycine, and 0.1% SDS.

Stripping Membranes:

Modification:

Article Title: Proteomic Signature of Fatty Acid Biosynthesis Inhibition Available for In Vivo Mechanism-of-Action Studies
Article Snippet: .. Modified according to Fujita et al. (9) with permission of the publisher. on July 17, 2012 by U N IV O F S U S S E X http://aac.asm .org/ D ow nloaded from rehydration for 18 h. Isoelectric focusing was carried out using a Multiphor II apparatus (GE Healthcare), applying the following gradient: 0 to 500 V for 1 kVh, 500 V for 0.02 kVh, 500 to 3,500 V for 3 kVh, and 3,500 V for 57 kVh at 20°C. ..

Electrofocusing:

Article Title: Proteomic analyses of age related changes in A.BY/SnJ mouse hearts
Article Snippet: Linear pH 4–7, 24 cm IPG strips (GE Healthcare) were rehydrated overnight in rehydration solution (urea 8 M, thiourea 2 M, pharmalyte 3–10 and 10 × dithiothreitol (DTT)) containing two labeled samples (Cy3 and Cy5, each 50 μg) and the corresponding internal standard (Cy2, 50 μg) for first dimension separation. .. The isoelectric focusing (IEF) was performed using a Multiphor II apparatus (GE Healthcare) with voltages ranging from 500 V to 3500 V for 17.50 h as suggested by the manufacturer. ..

other:

Article Title: Differential protein expression in Colletotrichum acutatum : changes associated with reactive oxygen species and nitrogen starvation implicated in pathogenicity on strawberry
Article Snippet: For measurement of ROS upon nutritional induction, 80 μL conidia (10 5 conidia/mL) were resuspended in Reg medium or in water containing 5 m m of one of the various nitrogen‐containing compounds tested (glutamine, urea, nitrate, nitrite or ammonium sulphate), and allowed to germinate in Microtest plates (Sarstedt, Inc., Newton, NC) for various times within a 16‐h period, before staining for 1 h with the H 2 DCFDA probe.

Review